Abstract: Objective To investigate monkeypox virus infection in lab animals in Beijing. Method Refer to the guidance of the World Health Organization ( WHO) for laboratory testing of monkeypox virus, a total of 642 animals in Beijing were detected by fluorescent PCR, including 127 monkeys, 215 mice, 80 rats, 40 guinea pigs, 10 hamsters, 85 rabbits and 85 dogs, Pharyngeal swabs were collected from monkeys, cecum was collected from rats, guinea pigs and hamsters, and anal swabs were collected from rabbits and dogs. The collected samples were added with appropriate amount of sterile PBS, The cecum samples were fully homogenized, the swab samples were swirled for 1 min, repeatedly freezethawed at -70 ℃ for 3 times, the supernatant was centrifuged to extract nucleic acid, and the monkeypox virus DNA was detected by fluorescent PCR, and the result were analyzed finally. Result 127 monkeys, 215 mice, 80 rats, 40 guinea pigs, 10 ground mice, 85 rabbits and 85 dogs were all negative for monkeypox virus DNA by fluorescent PCR. Conclusion Monkeypox virus infection has not been found in lab animals in Beijing at present.

Key words: lab animal, monkeypox virus, fluorescent PCR

摘要： 目的 对北京地区实验动物中猴痘病毒( MPXV) 感染情况进行调查。 方法 参照世界卫生组织 ( WHO) MPXV 实验室检测指导意见,采用荧光 PCR 法检测北京地区实验动物中 MPXV,共 642 只动物,包括猴 127 只、小鼠 215 只、大鼠 80 只、豚鼠 40 只、地鼠 10 只、兔 85 只、犬 85 只。 其中猴采集咽拭子,大小鼠、豚鼠和地鼠等采集盲肠,兔和犬采集肛拭子,采集的样本加入适量灭菌 PBS,盲肠样本充分匀浆,拭子样本涡旋振荡 1 min,-70 ℃ 反复冻融 3 次,离心取上清提取核酸,荧光 PCR 检测 MPXV DNA,最后对结果进行分析。 结果 经荧光 PCR 检测,127 只猴、215 只小鼠、80 只大鼠、40 只豚鼠、10 只地鼠、85 只兔、85 只犬 MPXV DNA 均为阴性。 结论 目前未在北京地区实验动物中发现 MPXV 感染。

关键词: 实验动物, 猴痘病毒, 荧光 PCR